| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2481 | Thermobifida fusca | TGTTCGGCTTCCTGTTCCC | ACGAGGTGGTCAATGGACG | 59.93 | 59.71 | 211 | 43 |
100.00%
|
100.00%
|
| Primer 2482 | Thermobifida fusca | TGCCGTGGATGTGGTTCA | AACATCAGCAGGACAGGCC | 59.17 | 60.00 | 166 | 43 |
100.00%
|
100.00%
|
| Primer 2483 | Thermobifida fusca | TGCCGTGGATGTGGTTCA | TGGAGGAGAGCAGCAGAGT | 59.17 | 59.92 | 273 | 43 |
100.00%
|
100.00%
|
| Primer 2484 | Thermobifida fusca | ATCGGGACACCCTACGACA | TGCAGTTCGTTGCCCTCA | 60.00 | 59.49 | 188 | 43 |
100.00%
|
100.00%
|
| Primer 2485 | Thermobifida fusca | TGGCGTTGCGGAAACTCA | AGCGTACTTGGCAGCGTA | 60.20 | 59.03 | 296 | 43 |
100.00%
|
100.00%
|
| Primer 2486 | Thermobifida fusca | GGGTCACCGCAGAATTCGA | GTGGAACTGCACGTTGTCG | 60.08 | 59.72 | 278 | 42 |
100.00%
|
100.00%
|
| Primer 2487 | Thermobifida fusca | GGGTCACCGCAGAATTCGA | GTGGTGGAACTGCACGTTG | 60.08 | 59.64 | 281 | 42 |
100.00%
|
100.00%
|
| Primer 2488 | Thermobifida fusca | TTTCAAGAGGGCAGCCGA | CCCCACAAAAGCAGCGTT | 58.84 | 58.87 | 259 | 42 |
100.00%
|
100.00%
|
| Primer 2489 | Thermobifida fusca | TTGAGCTCACCAACGCGA | CGTCCACCTGCTCTTTGGT | 59.58 | 59.93 | 270 | 42 |
100.00%
|
100.00%
|
| Primer 2490 | Thermobifida fusca | CCACTCTGTTCGAAGGCGA | CAGCTCCAGCACTTCGACA | 59.71 | 60.01 | 155 | 42 |
100.00%
|
100.00%
|